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Description

Mechanisms of endocochlear potential generation by stria vascularis

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Long-term 3D PureCol EZ Gel (Fujii medium) Ileum (N39) organoids were cultured in expansion medium consisting of DMEM/F12 supplemented with 100 U ml 1 P/S, 10 mM HEPES, 1 Glutamax, 2% B-27 with VitA, 3% (v/v) recombinant R-spondin-3, 2% (v/v) recombinant noggin, 1 mM N -acetylcysteine, 0.5 nM Wnt surrogate, 0.1 g ml 1 hIGF-1 (Peprotech), 0.05 g ml 1 hFGF2 (Peprotech), 10 nM gastrin, 0.5 M A83-01, 0.05 g ml 1 hEGF, 3 M CHIR 99021 and 10 M forskolin (Tocris) 42

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In addition to the kynurenine pathway, NAD + can also be generated from a salvage pathway 50

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10.1016/s0002-9378(00)70233-x 43 DeRuiterM

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