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Isolation of primary hepatocytes Primary mouse hepatocytes were obtained using a modified version of a two-step collagenase perfusion technique described by Seglen [28]

c-MYC Amplification of c-MYC and activation of its downstream effectors are related with high metastatic ability, endocrine resistance and poor disease outcome in breast tumors (179)

Next, we evaluated the role of SIRT1 in protection against NIHL by exposing both SIRT1 CKO and SIRT1 flox/flox mice to noise at 108 dB SPL for 2 h and assessing hearing thresholds after 14 days (Fig