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As a positive control for CytoID staining, d2 female flies were fed for 7 days on standard SYA medium supplemented with 200 M rapamycin (R-5000, LC Laboratories), then 48 h on a diet containing 200 M rapamycin and 10 mM chloroquine prior to gut dissection and staining as described above

Total protein, not surprisingly, measures the total protein levels in your blood

Colombo N, Bini S, Miceli D, et al Weekly cisplatin +/- glutathione in relapsed ovarian carcinoma

This causes depolarization of the mitochondrial membrane and the opening of the mitochondrial permeability transition pore (MPTP) and decreased ATP production