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Macrolide This 5-month-old boy presents with a 10-day history of severe paroxysmal coughing spells followed by an inspiratory gasp and post-tussive emesis

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disrupted by a three freezing-thawing cycles in liquid nitrogen and hot water bath and strong mixing (Vibrax VXR, Ika) for 10 min at 4C

Results are very robustin worst case exclusion sensitivity analysis 17 of 25 studies must be excluded before statistical significance is lost