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After that collect and rapidly wash the organ(s)/tissue with ice-cold saline

Shaking also creates foam, which dramatically increases the surface area exposed to air and can physically denature proteins and larger peptides through the mechanical forces at bubble interfaces

Moreover, IL-6 has new potential applications in COPD-related neuroinflammation, as it has been observed that elevated IL-6 can impair BBB integrity, increase the transfer of cytokines into the CNS, and stimulate microglia through JAK2-STAT3 pathways, leading to issues of synaptic function, neuronal apoptosis, and brain damage [23, 33]

Follow-up consultations can be arranged if needed