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Age Myelination is tightly regulated over the lifespan, with onset in humans in the third trimester and peaking in the first decade, and continuing on in adulthood to support learning and memory processes 86

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing