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ROS levels in these cells were assessed using DCFH-DA staining followed by fluorescence microscopy, revealing that BV2-metformin-loaded PLGA reduced intracellular ROS compared to uncoated nanoparticles and restored mitochondrial membrane potential (MMP) via JC-1 staining

doi: 10.1016/j.jbc.2022.101902, 99 PengS.ChenY.WangR.ZhangJ

Nemeth C, Humpeler S, Kallay E, Mesteri I, Svoboda M, Rgelsperger O, Ekmekcioglu C (2011) Decreased expression of the melatonin receptor 1 in human colorectal adenocarcinomas

One consequence of metabolic reprogramming is that some cancer cells become dependent on metabolic substrates including glucose and glutamine for survival 6, 7