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Direct cardiac effects of SGLT2 inhibitors

SOCE were triggered by depleting Ca 2+ from the ER with cyclopiazonic acid (10 M, CPA), a reversible SERCA (Sarcoplasmic and Endoplasmic Reticulum Ca 2+ ATPase) pump blocker, in Ca 2+ -free medium, and revealed by reinstating extracellular calcium concentration at 2 mM (Figure 2C)