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Description

Lancet 356:233241 Rayman MP (2002) The argument for increasing selenium intake

The mobile phase consisted of water with 0.1% formic acid (solvent A) and acetonitrile with 0.1% formic acid (solvent B), with a gradient elution program: 01.5 min at 2% B, 1.59 min with a linear gradient from 2% to 50% B, 914 min with a linear gradient from 50% to 95% B, and 1417 min at 95% B, all at a flow rate of 300 l/min