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A tumours metabolic requirements and their regulation can be changed upon cell adaptation to in vitro culture conditions, where the concentrations of glucose and glutamine are significantly higher than physiological, whereas other components are quite often missing

These cytokines also increase the production of matrix metalloproteinases (MMPs) and receptor activator of nuclear factor kappa-B ligand (RANKL), which leads to osteoclastogenesis and osteoclast activation, which deteriorates bone and cartilage in joints (44, 45)