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hence, ESM1 has been established as a potential marker for endothelial dysfunction (4,16,17)

Viable hepatocytes were resuspended in Hepatocyte Medium (Williams E, 5% FBS, 10 nM insulin, 100 nM dexamethasone, 100 nM triiodothyronine, and 100 g/mL penicillin-streptomycin), or, for Mpc fl/fl and liver-specific knockout ( Mpc2 LKO ) hepatocytes, in high glucose DMEM, 10% FBS, penicillin-streptomycin, and 0.5 g/ml amphotericin B, and plated on collagen-coated tissue culture plates

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All marsupial species and monotremes tested have been able to metabolise glucose to lactate and glucose appears to be a good substrate for erythrocyte energy production in native animals