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Agarose beads are microspheres of agarose gels with different particle diameters and concentrations

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Total ROS and mtROS measurements Total ROS and mtROS were measured using the fluorescent probes H2DCFDA (Thermo Fisher Scientific, Waltham, MA, USA) and MitoSOX (Thermo Fisher Scientific, Waltham, MA, USA), respectively

178 In addition, the secreted Cu-binding glycoprotein SPARC (secreted protein acidic and cysteine-rich, also known as osteonectin) has been shown to modulate cellmatrix interactions and promote the invasion and metastasis of tumor cells