We also monitored weight gain or loss on the standard lab chow (control) and the ketogenic diet (Figure 1C), finding no significant differences across sex or group
While Ub 4 -Eos3.2-tail is robustly degraded with or without NUB1, Ub 4 -Eos is not
1,25 VD3 from Sigma-Aldrich (CAS # 128723-16-0), BCA protein quantitation kit (Boster Bio, Cat # AR0146), RIPA lysis buffer (Boster Bio, Cat # 0105), protease inhibitor cocktails (Boster, Bio Cat # AR1182), phosphatase inhibitor (Boster Bio, Cat # AR1183), color pre-dyed protein marker (Boster Bio, Cat # AR1113), Western-specific primary and secondary antibody diluent (Boster Bio, Cat # AR1017), wash buffer TBS-T (Boster Bio, Cat # AR0195-10), ECL chemiluminiscent reagent (Boster Bio, Cat # AR1196), BSA TBS buffer system blocking solution (Boster Bio, Cat # AR0189), NF-B antibody (Boster Bio, Cat # A01228-1), -actin antibody (Boster Bio, Cat # M01263), HRP-conjugated goat anti-rabbit IgG (Boster Bio, Cat # BA1054), CD163 antibody (Boster Bio, Cat # A00812-2), CD11c antibody (Boster Bio, Cat # A00357-3), CD68 antibody (Boster Bio, Cat # BA3638), fluorescent (DyLight 488) labelled goat anti-rabbit IgG (Boster Bio, Cat # BA1127), fluorescent (DyLight 594) labelled goat anti-rabbit IgG (Boster Bio, Cat # BA1142), rat TNF- ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0180R1), rat IL-1 ELISA kit (Boster Bio, Cat # EK0393), rat IL-4 ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd, Cat # MM-0191R1), rat IL-10 ELISA kit (Boster Bio, Cat # EK0418), 25 VD3 assay kit (Roche Diagnostics, Cat # 07028148190), TAOC assay kit (Nanjing Jiancheng, Cat # A015-3-1), MDA assay kit (Nanjing Jiancheng, Cat # A003-1-2), and free fatty acid kit (Kunchuang Biotechnology, Xian, China, Cat # SK125-2)
BCL-2, the proto-oncogene, has the ability to block apoptosis, leading to reduced cell death and an enhanced proliferation (Westphal et al., 2014)
