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Beyond their established use in sleep disorders, ramelteon alleviates experimental acute ocular inflammation via HIF-1/VEGF/E-NOS signaling (Usta Sofu et al., 2022), while agomelatine attenuates cisplatin-induced nephrotoxicity by suppressing NF-B-driven inflammation ( These advances are supported by systematic pharmacological reviews from our team and others (Ruan et al., 2021

For immunoblotting, the membranes were incubated with primary antibodies at 4 C overnight and then with secondary antibodies for 1 h at room temperature

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In addition, acidosis activates ASOR/PAC, which is a ubiquitously expressed anion channel (Okada et al., 2021b)