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Immunohistochemical analysis of drug resistance-associated proteins in ovarian carcinomas

In this study, we sought to determine how genetic ablation of the circadian clock in adipocytes exerts effects beyond the dysregulation of diet-induced thermogenesis and creatine cycling 12,13

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Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols