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Presynaptic cytoplasmic Ca 2+ can be depleted by several complementary mechanisms, including sarcoendoplasmic reticulum Ca 2+ -ATPase (SERCA) to the endoplasmic reticulum (ER), plasma membrane Ca 2+ -ATPase to the extracellular space, and MCU to the mitochondrial matrix [51,52,53]

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Clinical diagnosis of organic acid metabolism disorders can be assisted by gas-liquid chromatography examination of organic acids and their derivatives in urine and blood, and other auxiliary diagnostic methods include electroencephalogram, cranial nuclear magnetic resonance, enzyme analysis, and genetic testing, etc

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