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Acknowledgements We thank Professor Qianfei Wang (Beijing Institute of genomics, Chinese Academy of Sciences) and Professor Qianben Wang (Department of Pathology, Duke University School of Medicine, Durham, USA) for critical comments and advice on the manuscript

For SureBeads, the beads were pre-incubated with the GFP antibody, washed, and then incubated with lysates for 1 h at 4 C with gentle agitation

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Deubiquitinated arrestins interact with G and enters the nucleus via importin complex, sequestering G from the receptor and G, which reduces receptor signaling efficiency (desensitization)