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4.6 Neuronal differentiation IMR-5/75 cells were plated in duplicates on a 6-well plate in 2 mL complete RPMI phenol red-free media (Gibco)

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Consider Alternatives If Who Should Avoid Do not use if You have Wilson's diseaseyour body already can't process copper properly, and adding more is dangerous You're allergic to copper or have had reactions to copper-containing products You have active cancerGHK-Cu stimulates cell growth and blood vessel formation, which could theoretically fuel tumor growth You're pregnant or breastfeedingnot enough safety data exists for these populations You have severe liver diseasethe liver processes copper, and impaired function could lead to toxic buildup You're under 18this is for adult use only in research settings Use with caution if !You take copper supplements alreadytoo much copper can be toxic, so monitor your total intake !You have a history of kidney diseasecopper is partially cleared through the kidneys !You're on blood thinnersmay increase bruising at injection sites !You have autoimmune conditionsGHK-Cu modulates immune function in ways that might be unpredictable !You take high-dose zinc supplementszinc and copper compete for absorption !You're planning surgery soonits blood vessel growth effects may complicate procedures Administration How do I use it

This boundary keeps the article aligned with laboratory research-use-only positioning