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People forget that these are not mutually exclusive, like meal frequency, If youre not eating a lot, intermittent fasting makes perfect sense

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A 200 ug/kg or 2 ug/kg dose of BPC 157 was intraperitoneally injected 10 minutes post-injury followed by the return of animals to their cages and assignment to interval groups varying from days 7, 15, 20, 90, 180, and 360

Then, lysates were heated with Laemmli sample buffer (final 1X composition: 20 mM Tris, pH 6.8, 2% sodium dodecyl sulfate (SDS), 6% glycerol, 1% -mercaptoethanol, 0.002% bromophenol blue) at 95 C for 5 min