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Although the use of Standard Seawater to determine Practical Salinity has been routine for many years, the dependence of Practical Salinity measurements on a physical artifact known to degrade with age leads to a number of technical problems, especially in terms of the long-term stability and intercomparability of high-precision ocean measurements

While nitrosation of enzymes and formation of S-nitro groups at their active centers was found to be potentially reversible with thiol agents that restored activity, the nitrosation of other proteins, and in particular nitrotyrosine formation, is pathogenic