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In the kidneys of STZ-induced diabetic mice and HK-2 cells cultured with high glucose, iron overload, decreased antioxidant capacity and high rates of ROS production and lipid peroxidation are the signature changes of ferroptosis [225, 228]

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Whenever -tubulin was co-labeled, the PFA-fixed cells were further treated with chilled methanol for 30 s at 20C

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