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Dual-luciferase reporter assay The mouse Hnf4a promoter sequences were inserted into the NheI/HindIII site of the luciferase pGL3-Basic vector (Promega, USA)

As predicted by Polly Matzinger (62, 223), DAMPs trigger the activation of antigen-presenting cells, act as chemokines that recruit dendritic cells, macrophages, or T cells to the site of injury, or prime immune cells, i.e., they contribute to trained immunity (5860, 62, 224226)

Peer review Peer review information Nature Reviews Neurology thanks Mu-Hong Chen, and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

11), recent X-ray crystallography and cryo-EM data revealed that GSH binds the ATD of GLR3.4 30 , another GLR protein with high sequence similarity to GLR3.3, reinforcing that GSH might bind the ATD domain of GLR3.3 for activation of ion channel