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Chaotropes should be avoided whenever possible since they are likely to denature the eluted protein

Skin Pharmacol Appl Skin Physiol 14:203209 Hikima T, Yamada K, Kimura T, Maibach HI, Tojo K (2002) Comparison of skin distribution of hydrolytic activity for bioconversion of -estradiol 17-acetate between man and several animals in vitro

Furthermore, unregulated status of zinc leads to oxidative stress, cell cycle progression, damage of DNA, and angiogenesis, finally resulting in hepatocarcinogenesis (71)