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Particle titers of purified viruses were quantified by making three separate dilutions of each virus in 0.1% SDS (Thermo Fisher) and vortexing for 5 minutes, spinning down 13,000 x rpm, 5 minutes, and then taking the average of the absorbance readings on the Nanophotometer N50-GO (Implen) to quantify particles/mL as per (Maizel et al., 1968)

J Neurochem 82:716719 Hirrlinger J, Moeller H, Kirchhoff F, Dringen R (2005) Expression of multidrug resistance proteins (Mrps) in astrocytes of the mouse brain: a single cell RT-PCR study

doi: 10.1128/aac.02284-19 120 FernandesK

The sequence consists of a T-rex domain linked with an SAAGGH amino acid sequence to circular permutated TS, followed by a single amino acid Thr linked to a second T-rex domain, a GSGTGGNASDGGGSGG linker, and the mCherry sequence