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Metformin attenuates hepatoma cell proliferation by decreasing glycolytic flux through the HIF-1/PFKFB3/PFK1 pathway

10.3389/fmed.2021.736978 189 StockwellB

Generation of Knockout Plasmids and Transfections Two different plasmids (pL0001 and pL0034) were used to create a total of four knockout plasmids (Figure 1) to attempt disruption of the pbgst gene