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After three days the media are removed, and the cells washed three times with phosphatebuffered saline (PBS) before the addition of serum-free Dulbecco's medium containing 0.25 mg/ml bovine serum albumin (BSA, Fraction V, Sigma Chemical, St

The analysis showed that the chestnut genome reference, as judged by number of shared orthogroups, does not significantly differ from other closely and more distantly related species