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Multiple comparisons were controlled using a more conservative Bonferroni corrected alpha level of 0.016 per test, ensuring a family-wise error rate of 0.05

Five DEGs were involved in the biosynthesis of 4 proteins required in the pathway of one-carbon pool by folate, among which the 5-formyltetrahydrofolate cyclo-ligase (MTHFS) has a scavenger role as the only enzyme that converts 5-formyl-THF to 5,10-methenyl-THF [46] and showed a value of log2FC 2.69 at 3 DPI vs CK (Additional file 1: Dataset S1)

As a supportive recovery step If the skin feels calmer but still texturally uneven, GHK-Cu may be reasonable as a support ingredient in a broader recovery routine

Handling Guidelines Proper handling practices help maintain peptide stability and reduce contamination risks in laboratory environments