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Fixed cells were washed twice in PBS containing 10% serum and stained with 50 g ml 1 propidium iodide solution containing 100 g ml 1 RNase A for 1 h at 37 C

Acta Sci Agri

However, slightly inadequate zinc intake is also observed in young children and the elderly over 69 years of age in developed countries (42, 52), suggesting that many factors interact to cause zinc deficiency (53)