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(4) Wash the column with approximately 10-15 bed volumes of Binding/Wash Buffer or until the absorbance is stable

Guo, G., Kan, M., Martinez, J

The mechanism attributed to this effect was decreasing oxidative stress, NFB activation, and inflammatory cytokine (TNF-, and IL-6) levels, secondary to Nrf2 activation and upregulation of phase 2 enzymes HO-1, GCL, and GST (Thangapandiyan and Miltonprabu, 2014)