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By inhibiting the phosphorylation and nuclear translocation of NF-B, scutellarein, on the one hand, reduces the transcription and secretion of pro-inflammatory factors such as IL-6, IL-8 and TNF-, on the other hand, upregulates the expression of tight junction proteins such as E-cadherin, Occludin and ZO-1 in colonic epithelial cells to maintain the integrity of the epithelial barrier

However, high molecular-weight aggregates are often detected by the anti-GST antibody after protein separation by SDS-PAGE

10.3390/plants9121672 129 KuttithodiA

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