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doi: 10.1016/j.jcmgh.2018.10.009 58 FordA

Bacterial cells were collected and lysed in STE buffer (10 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM EDTA) containing 0.1% Triton X-100, 100 g/mL lysozyme, 1 mM PMSF, and 1X PIC

These simulations revealed the location where the receptor binding domain attaches to the ACE2 receptor a stretch of the ACE2 protein that forms a structure called an alpha helix