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[111] These measurements, however, are currently unavailable for some of the most stable SOD1 mutants (L117V), and represent important areas for future investigation given they may underlie misfolding or aggregation of these more perplexing mutant proteins

Single stranded cDNA was synthesized from total RNA isolated from sham, TBI, GSNO, and SIN-1-treated brains at 24 h and 14 days following TBI using the superscript pre-amplification system for first strand cDNA synthesis (Invitrogen, Carlsbad, CA) as described earlier [63]