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Gutman made another career shift, committed to advancing glutathione research, eventually becoming both the personal physician of and trusted collaborator with Dr

Cell viability Cell viability was assessed by a colorimetric assay where MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 0.5 mg/ml

If youre working with an uncharacterized protein, or a protein for which a good antibody has not been developed (and just because your protein has a commercially available antibody, that doesnt mean its a good one), then your first step towards detecting, immunoprecipitating, or purifying that protein may be to fuse an affinity tag to it

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