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Subsequently, correct insertion of the mutant gene fragments into the marker free gshA mutant were checked using appropriate forward and reverse primers of the respective genes in combination with the k1 and k2 KEIO primers listed in Table 3 using colony PCR

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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Wasylenko TM, Ahn WS & Stephanopoulos G The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica