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Once the needle is in the muscle, the plunger is pulled back slightly to ensure the needle is not in a blood vessel

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Concurrently, it is imperative to disentangle the concrete regulation on functional subgroups of the gut microbiota induced by metabolites from articular ferroptosis (for example, ROS and LPO) (such as probiotic genus composition, abundance of populations such as those of Lactobacillus and the Bacteroidetes/ Firmicutes ratio) and gut structural mucosal barrier integrity (for example, the expression of tight junction proteins, such as occludin and claudin)