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In addition, CRISPR-engineered reporter systems, such as tyrosine hydroxylase (TH)-linked fluorescent tags and lineage tracers, enable real-time visualization, purification and longitudinal tracking of DA neurons while preserving endogenous regulation, which is crucial for high-content phenotyping and enables the precise assessment of drug effects and screening 43,44,45,46

anti-sense, 5-GCAACGTGCAGCCGGAGATAAGACCCGGCTCTAGAGG-3) in DNA binding buffer (0.3 mg/ml bovine serum albumin (BSA), 0.1 mg/ml salmon sperm, 20 mM Hepes pH 7.9, 1.5 mM MgCl 2 , 400 mM NaCl, 0.2 mM EDTA, 25% glycerol, 0.02% Tween10) and incubated for 2 h at 4C

doi: 10.1111/bph.15930 245 CarabottiMSciroccoAMaselliMASeveriC