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Wavelength selection by ink colour is non-negotiable, using the wrong wavelength either fails to fragment the pigment (waste of session) or causes paradoxical darkening (particularly with iron-containing pink and red inks, or with white/flesh-coloured inks used in cover-ups)

Membranes were blocked for non-specific binding via incubation in 0.1% PBS-Triton X-100 containing 5% Albumin bovine fraction V [BSA] (Cat