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This depends on the level of melasma pigment

Estrogen, which is typically higher in females, may suppress testosterone synthesis (24), and vitamin B12 might amplify this effect by enhancing estrogen activity or synthesis (25)

Materials and Methods Proteins and reagents Recombinant human Trx1 and Grx1, rat recombinant TrxR and polyclonal goat anti-Trx1 antibody were obtained from IMCO Corporation, Sweden (www.imcocorp.se)