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In Vitro AAPH (600 M, 0-1 h) oxidates DCFH to form a fluorescent substance, DCF, which the level of fluorescence was proportional to the degree of oxidation in HepG2 cells [2]

(Phila.) 55 (10), 10551065 (2017)

(2001) 281:L18392

The PPP is the major source of NADPH, which is a redox cofactor for ROS production via nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase and is required for both the generation of the antioxidant glutathione and lipid synthesis ( Fatty acid metabolism, including FAO and FAS, is composed of anabolic and catabolic processes that generate diverse metabolic intermediates to facilitate energy storage, the biosynthesis of membrane lipids, and the production of signaling molecules ( de novo synthesis of fatty acids, carbon atoms from glucose or amino acids are converted to fatty acids, which tightly couple glucose and lipid metabolism ( De novo FAS is suggested to fuel FAO in M2 macrophages ( Amino acid metabolism also has an important influence on macrophage polarization