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The selection criteria of the study were constrained to the language English because of the language barrier, high cost of translation, and time efficiency

Immunofluorescence against the astrocyte marker glial fibrillary acidic protein (GFAP) and the microglial marker ionized-calciumbinding adaptor molecule 1 (Iba-1) was evaluated in coronal cryosections of hippocampus (30 m thick) as previously reported (Ezquer et al., 2 , and the thickness ( z axis) was measured for each case