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SMD treatment in a previous study improved performance assessed using the Morris Water Maze and Y-Maze test in mice exposed to IH by enhancing ERK/CREB phosphorylation and elevating PSD-95 and BDNF expression (Zhao et al., 2021)

Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

doi: 10.1002/(SICI)1096-9888(200002)35:2 3.0.CO;2-A

But if the good versus evil axis gets out of whack and there are more free radicals in the body than antioxidants, it results in a condition called oxidative stress