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[10] Its administration during pregnancy carries potential risks to fetal development, and it is not indicated for use once pregnancy is confirmed

Widespread siRNA off-target transcript silencing mediated by seed region sequence complementarity

doi: 10.1016/0006-2952(71)90202-4

3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work