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L27W mutation did not further increase the affinity of rat amylin(2737) for amylin receptor 2/3 ECD compared to L27Y mutation (Figure 6E, 6F and Table 2)

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At concentrations of 1 pM1 nM, DSIP stimulated calcium-dependent release of immunoreactive Met-enkephalin from rat lower brainstem slices in vitro suggesting any antinociceptive effects observed in animal models may be mediated through endogenous opioid peptide release rather than direct receptor binding [7]