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For neuroprotective agents like this compound, it is essential to employ multiple complementary assays that evaluate different aspects of neuronal health, including metabolic activity, membrane integrity, and morphological features

IGF-1 signaling is essential for terminal osteoblast differentiation of MSCs, whereas the recruitment of MSCs to the bone remodeling surface was not significantly affected

Concentration of pure compounds in Standard stocks were validated by UV-VIS spectroscopy as described elsewhere

Terminal labeling contralateral to injury: polyethylene glycol-fused animals Rostrocaudal extent At all post-operative timepoints following either Single Cut or Allograft PEG-fusion repair, animals reliably exhibited terminal labeling contralaterally (29 out of 29 cases) within the correct boundaries around the L3/L4 segmental division established in Unoperated Control animals ( Figure 7A )