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Cultures were split at this stage and treated with subinhibitory concentrations of EPA (2 g/mL), or GLA (2 g/mL), LNA (3 g/mL), OA (3 g/mL), or SA (3 g/mL)

In some cells, ferroptosis acts as an active driver of tissue destruction, whereas in others, it represents a passive consequence or an accompanying phenomenon of the pathological environment

Specifically, they demonstrated that lactic acid can modify histones, thus influencing gene transcription, and, for the first time, identified lactic acid as an epigenetic regulator. Subsequent studies revealed widespread lactylation across various cell types, involving both histone and nonhistone lactylation, implicating this modification in signal transduction, metabolic regulation, and cell fate determination

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