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Cells were washed with PBS, stained with APC anti-mouse Thy1.1 antibody (1:400, 202526, BioLegend) and Zombie NIR Fixable Viability Dye (1:1000, BioLegend) in the presence of Human TruStain FcX Fc Receptor Blocking Solution (1:400, 422302, BioLegend) for 10min at 4C in the dark, and fixed with 1mL BD CytoFix Fixation Buffer (BD Biosciences, 554655) for 30min at 4C in the dark