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Flow cytometry revealed a significant increase in IL-24 cells in the lungs of HDM-challenged mice compared to nave controls (Supplementary Fig

The resulting solution was clarified of cell debris by centrifugation and analyzed for GSH levels or GCL activity

Unlike drinking coffee, this method allows compounds like caffeine, theobromine, and palmitic acids to be absorbed through the rectal blood vessels and delivered more directly to the liver

To test this, we stained cells with fluorescent dyes labelling cytoplasm or plasma membrane and quantified the flat, protrusive peripheral parts of the cell using a custom-made image analysis pipeline (Methods