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Figure 1b shows the resulting temperature change for the pump modulation frequency of f = 1 kHz and n = 2

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ROS have a significant effect on the sperm plasma membrane and subsequent functional integrity of the sperm resulting in a loss of acrosome reaction [11], the sperm potential for fertilization [12], sperm motility and vitality [13, 14] as well as impair the sperm capacitation [15] (Figure 1)