When MGSLCSR was introduced into the full-length Go, however, the cytosolic localization of this mutant was well visible (Supplementary Fig
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Microspheres not used for biological experimentation were washed with DI water, while those used in biological applications were further washed with Dulbeccos Phosphate-Buffered Saline (DPBS, acquired from Sigma Aldrich, catalog number P3813), was used instead of normal PBS to avoid residual crosslinking from calcium and magnesium ions present in regular PBS, which is known to interfere with the alginate hydrogel matrix 49
