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Premedication was administered using 3 mg/kg of xylazine, followed by general anesthesia with 50 mg/kg of ketamine

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On the next day, the cells were treated with NCs containing eGFP-siRNA (sieGFP) and negative control-siRNA (siCTRL) (264 nM siRNA concentration) for 4 h at 37C in RPMI